Porton Advanced is introducing a new CRISPR gene editing service for stable cell line construction and stable cell line engineering. Our new CRISPR editing technology will provide customers with fast, accurate, and integrated technical services. We offer customized editing solutions suitable for various cell lines, while also supporting diverse delivery methods such as RNP, lentivirus, plasmids, and multiple detection methods for basic research, antibody validation, drug candidate screen, disease diagnosis, and etc.
Porton Advanced has built CRISPR, a gene editing platform with proven results. We are able to formulate CRISPR based methodologies by implementing gene editing techniques including knock-in/knock-out in varying cell lines as per client request. The knock-in efficiency can be over 40%, while the knock-out efficiency can be over 98%.
Our experts have extensive experience and a deep understanding in viral transfection/non-viral electroporation systems. By making continuous efforts in optimizing the procedures, our team guarantees a high-efficient and stable transfection/electroporation system with high cell viability.
For certain targets, our experts use dual test efficiency at the nucleic acid and protein levels to assess gene editing efficiency, whereas cell viability assay is used for deliverables control. Our testing services fully assess gene editing efficiency by conducting testing from aspects of cell characteristics, gene expression regulation, and cell function.
Our project management can customize experimental plans with optional deliverables and routinely report project progress, per client request.
We offer customized editing solutions suitable, parameters optimization and gene editing efficieny testing for target gene, area or applications.
With advanced technology and expertise in gene editing, our team develops customized schemes based on the customer’s specified target gene, editing region, or downstream production goals. The scheme includes target site selection, editing system, detection methods, and more.
Multiple databases are used for comprehensive analysis of the target site sequence, for determining sgRNA sequences, for predicting editing efficiency and for potential off-target sites. Additionally, donor DNA, or relevant plasmid design and construction required for gene editing experiments, is provided.
The appropriate delivery method for the editing system is selected based on the customer’s experimental and subsequent production needs, including viral based (LV, AAV) transfection, plasmid, or RNP electroporation delivery. Optimization of transformation parameters and efficiency testing services are also available.
Gene editing efficiency validation includes detection of editing efficiency at the gene and/or protein level, as well as off-target analysis (outsourced).
Cas9/cas12 (knock-out/knock-in), CRISPRi/a (silencing/activation), Base Editor (base editing), Primer Editor (primer editing), and Epigenetic Regulation.
With highly efficient and stable cell transfection and gene editing systems, Porton Advanced has successfully achieved gene editing in different cell lines, including but not limited to T cells, TIL cells, NK cells, iPSCs, and HSPCs.
Gene editing design scheme, sgRNA and Donor DNA, sequences and synthesized products, optimization of parameters for gene editing system delivery, and an efficiency testing report.