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CRISPR Gene Editing Service

Porton Advanced is introducing a new CRISPR gene editing service for stable cell line construction and stable cell line engineering. Our new CRISPR editing technology will provide customers with fast, accurate, and integrated technical services. We offer customized editing solutions suitable for various cell lines, while also supporting diverse delivery methods such as RNP, lentivirus, plasmids, and multiple detection methods for basic research, antibody validation, drug candidate screen, disease diagnosis, and etc.

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Gene Editing Strategies and
Efficiency Validation
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Stable Cell Line
Engineering
Porton Advanced offers end-to-end CRO & CDMO service with seamless technical transfer. Click here for in vivo/in vitro CDMO

Why Choose Us?

Mature Technology Platform

Porton Advanced has built CRISPR, a gene editing platform with proven results. We are able to formulate CRISPR based methodologies by implementing gene editing techniques including knock-in/knock-out in varying cell lines as per client request. The knock-in efficiency can be over 40%, while the knock-out efficiency can be over 98%.

High-efficient Transfection System

Our experts have extensive experience and a deep understanding in viral transfection/non-viral electroporation systems. By making continuous efforts in optimizing the procedures, our team guarantees a high-efficient and stable transfection/electroporation system with high cell viability.

Comprehensive Testing System

For certain targets, our experts use dual test efficiency at the nucleic acid and protein levels to assess gene editing efficiency, whereas cell viability assay is used for deliverables control. Our testing services fully assess gene editing efficiency by conducting testing from aspects of cell characteristics, gene expression regulation, and cell function.

Professional Project Management

Our project management can customize experimental plans with optional deliverables and routinely report project progress, per client request.

Engineering

Gene Editing Strategies and Efficiency Validation

We offer customized editing solutions suitable, parameters optimization and gene editing efficieny testing for target gene, area or applications.

Introduction

Gene Editing Scheme design

With advanced technology and expertise in gene editing, our team develops customized schemes based on the customer’s specified target gene, editing region, or downstream production goals. The scheme includes target site selection, editing system, detection methods, and more.

sgRNA and Donor DNA Design

Multiple databases are used for comprehensive analysis of the target site sequence, for determining sgRNA sequences, for predicting editing efficiency and for potential off-target sites. Additionally, donor DNA, or relevant plasmid design and construction required for gene editing experiments, is provided.

Gene Editing System Delivery

The appropriate delivery method for the editing system is selected based on the customer’s experimental and subsequent production needs, including viral based (LV, AAV) transfection, plasmid, or RNP electroporation delivery. Optimization of transformation parameters and efficiency testing services are also available.

Gene Editing Efficiency Validation

Gene editing efficiency validation includes detection of editing efficiency at the gene and/or protein level, as well as off-target analysis (outsourced).

Workflow

A diagram showing the process of making a dna.

Based on the CRISPR/Cas nucleases gene editing system

Cas9/cas12 (knock-out/knock-in), CRISPRi/a (silencing/activation), Base Editor (base editing), Primer Editor (primer editing), and Epigenetic Regulation.

Various Cell Applications

With highly efficient and stable cell transfection and gene editing systems, Porton Advanced has successfully achieved gene editing in different cell lines, including but not limited to T cells, TIL cells, NK cells, iPSCs, and HSPCs.

Deliverables

Gene editing design scheme, sgRNA and Donor DNA, sequences and synthesized products, optimization of parameters for gene editing system delivery, and an efficiency testing report.