A blue background with many cells in it.

Pre-made and Custom-Made mRNA and circRNA

Introduction

mRNA therapeutics hold the potential to transform modern medicine by providing a CGT platform with the capacity for rapid development and wide-scale deployment. Compared to recombinant proteins expressed in mammalian cell lines, manufacturing of mRNA is faster and more flexible as it can be easily produced by in vitro transcription.

Porton Advanced provides codon optimization, custom-made mRNA, custom-made circular RNA, and premade mRNA with options: capping, modified nucleotides, or lipid nanoparticle (LNP). Our codon optimization includes comprehensive consideration of RNA secondary structure, GC content and distribution, repeated sequences, and codon usage frequency. Our team of experts is dedicated to collaborating closely with you, ensuring that every RNA molecule we provide aligns seamlessly with your research objectives.

Our circRNA molecules are meticulously crafted to accelerate your research breakthroughs. Unlock the potential of circRNA and illuminate new pathways in your scientific inquiries. Our ready-to-use IVT mRNA can be used as a control for your mRNA experiment, or to assess the efficiency of mRNA delivery systems. For research-grade custom IVT mRNA preparation, please check our CRO services below. For scalable manufacturing of mRNA drugs, please check out our CDMO services.

Product

Custom-made mRNA with the option of Capping, Modified Nucleotides, or Lipid Nanoparticles (LNPs)

Experience the epitome of precision with our custom-made mRNA solutions. Tailor your experiments with advanced capping (Cap1) and modified nucleotide options (N1-Methylpseudouridine-m1Ψ, 5-methylcytosine-m5C, etc.) or Lipid Nanoparticle (LNP), enhancing mRNA stability and translational efficiency.

Custom-Made circRNA

Delve into the world of circular RNA with our custom-made circRNA products. Circular RNAs have emerged as pivotal players in gene regulation, and our circRNA solutions are engineered to empower your investigations.

Pre-made mRNA with the option of capping, Modified Nucleotides

Our premade mRNA (EGFP/ mCherry/ Luciferase/ Cas9) was generated with cap1 and with or without modified nucleotide, N1-Methylpseudouridine (m1Ψ). Both the protein expression (EGFP/ mCherry/ Luciferase) and the editing function (Cas9) of our pre-made mRNA have been fully validated for high levels in the cell culture.

Product Nucleotide Scale
EGFP IVT mRNA with cap1 Unmodified 100 ug,500 ug,1 mg
m1Ψ substitution 100 ug,500 ug,1 mg
mCherry IVT mRNA with cap1 Unmodified 100 ug,500 ug,1 mg
m1Ψ substitution 100 ug,500 ug,1 mg
Luciferase IVT mRNA with cap1 Unmodified 100 ug,500 ug,1 mg
m1Ψ substitution 100 ug,500 ug,1 mg
Cas9 IVT mRNA with cap1 Unmodified 100 ug,500 ug,1 mg
m1Ψ substitution 100 ug,500 ug,1 mg
 EGFP circRNA Unmodified 100 ug,500 ug,1 mg
 mCherry circRNA Unmodified 100 ug,500 ug,1 mg
 Luciferase circRNA Unmodified 100 ug,500 ug,1 mg

mRNA Case study

Porton Advanced’s Analytical platform utilized the CGE method for assessing mRNA integrity. Results showed a high level of mRNA intactness, meeting the process development standard.
(source: Porton Advanced’s Analytical Development Platform)
Porton Advanced’s Analytical platform utilized the CGE method for assessing mRNA integrity. Results showed a high level of mRNA intactness, meeting the process development standard.
(source: Porton Advanced’s Analytical Development Platform)
Porton Advanced’s analytical platform showed the results on particle size distribution of LNP sample by SEC-MALS to be less than 0.1, proving the stable process delivered by the process development team.
(source: Porton Advanced’s Analytical Development Platform)

Workflow

A diagram showing the process of making a vaccine.

Quality control (QC)

Attribute Method Research Grade
General Appearance Visual inspection
pH Potentiometric analysis Optional
Identity mRNA length AGE
mRNA sequence Sanger sequencing
Content mRNA concentration UV spectrophotometry
Purity  A260/A280 UV spectrophotometry
mRNA integrity AGE
CGE
Capping efficiency LC-MS Optional
PolyA tail length,distribution LC-MS Optional
Impurity Residual DNA template qPCR Optional
 Residual protein Micro BCA Optional
dsRNA ELISA Optional
Residual cap analog RP-HPLC Optional
Residual NTPs RP-HPLC Optional
Potency Gene expression Western blot Optional
Cell transfection Optional
Safety Endotoxin Gel-clot / Photometric technique Optional
Sterility Membrane filtration Optional

Deliverables

  • IVT mRNA Product
  • Certificate of Analysis (CoA)

Storage

Our mRNA products are stored in Tris buffer (pH 7.4) and can be stored at -80°C for up to 12 months. Both products are shipped on dry ice and should not be subjected to frequent freeze-thaw cycles.