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Vector Cloning & Plasmid Preparation

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Vector Cloning Service

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Plasmid Preparation Services

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Engineering

Introduction

Porton Advanced has developed plasmid DNA preparation services, designed to provide rapid and cost-efficient delivery of high-quality DNAs. Our plasmid preparation services cover a broad spectrum, accommodating both scientific researchers and industrial clients, with quantities ranging from micrograms to grams. Our offerings not only to research-oriented projects but also extend to manufacturing GMP-like and GMP-grade plasmid DNAs suitable for preclinical and clinical applications. With a commitment to excellence, we are proud to meet the diverse needs of our customers in the academic, biotech, and pharmaceutical communities.

Services

Scale Application Deliverable(Plasmid DNA) Time
Miniprep Molecular biology 25 µg, >100 ng/µL 1 – 2 days
Midiprep Molecular biology and cell culture (endotoxin-free, sterile) 350 µg, >500 ng/µL 3 – 4 days
Maxiprep 1.2 mg, >500 ng/µL
Megaprep 5 mg, >500 ng/µL
Gigaprep 15 mg, >500 ng/µL 5 – 8 days
Industrial Grade Research and preclinical applications 20 mg to 1 g 1 – 2 weeks

Workflow (Maxiprep)

Day 1-2Plasmid transformation into E.coli and bacterial fluids preparation for plasmid extraction
Day 3Extraction and purfication of plasmid
Day 4-5Quality control
A diagram showing the process of using a syringe.

Quality Control (QC)

Our comprehensive quality control (QC) for plasmid DNAs includes DNA quantification, A260/280 and A260/230 measurements, restriction enzyme digestion, Sanger sequencing. For Maxiprep and above, our plasmid preparation services can achieve higher supercoiled content (90% ± 10%) with lower endotoxin levels (<0.01 EU/μg). Additionally, we offer animal-free plasmid preparation upon request.

Deliverables

  • Plasmid DNAs
  • Certificate of Analysis (CoA)
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Storage Condition

Our plasmid DNAs can be formulated in either 1 × TE buffer or ultra-purified HO. Formulated plasmid DNAs can be stored at -80°C for a period of 12-24 months without compromising plasmid DNA quality.