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CRISPR Gene Editing Service

Porton Advanced is introducing a new CRISPR gene editing service for stable cell line construction and stable cell line engineering. Our new CRISPR editing technology will provide customers with fast, accurate, and integrated technical services. We offer customized editing solutions suitable for various cell lines, while also supporting diverse delivery methods such as RNP, lentivirus, plasmids, and multiple detection methods for basic research, antibody validation, drug candidate screen, disease diagnosis, and etc.

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Gene Editing Strategies and
Efficiency Validation
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Stable Cell Line
Engineering
Porton Advanced offers end-to-end CRO & CDMO service with seamless technical transfer. Click here for in vivo/in vitro CDMO

Why Choose Us?

Mature Technology Platform

Porton Advanced has built CRISPR, a gene editing platform with proven results. We are able to formulate CRISPR based methodologies by implementing gene editing techniques including knock-in/knock-out in varying cell lines as per client request. The knock-in efficiency can be over 40%, while the knock-out efficiency can be over 98%.

High-efficient Transfection System

Our experts have extensive experience and a deep understanding in viral transfection/non-viral electroporation systems. By making continuous efforts in optimizing the procedures, our team guarantees a high-efficient and stable transfection/electroporation system with high cell viability.

Comprehensive Testing System

For certain targets, our experts use dual test efficiency at the nucleic acid and protein levels to assess gene editing efficiency, whereas cell viability assay is used for deliverables control. Our testing services fully assess gene editing efficiency by conducting testing from aspects of cell characteristics, gene expression regulation, and cell function.

Professional Project Management

Our project management can customize experimental plans with optional deliverables and routinely report project progress, per client request.

Engineering

Stable Cell Line Engineering

We offer customized editing solutions suitable for various cell scenarios, while also supporting diverse delivery methods such as RNP, lentivirus, plasmids, and multiple detection methods. This ensures efficient and stable cell lines in addition to comprehensive testing services.

Services

Gene knock-out stable cell line construction, gene knock-in stable cell line construction, and point mutation stable cell line construction.

Gene Editing Design

  • Customized editing schemes provided through multi-dimensional evaluation
  • Design of sgRNA, donor DNA, and relevant vectors

Cell Culture and Transfection

  • Applicable to various cell scenarios
  • Diverse delivery methods

Identification and Detection of Transfected Cells

  •  Multi-dimensional evaluation of editing efficiency
  • Multi-angle detection of cell function

Cell Line Delivery

  • Various forms of delivery such as RNP, lentivirus, plasmids, and multiple detection methods
  • Detailed delivery reports

Workflow

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CRISPR/Cas nucleases Gene Editing System

Cas9/cas12 (knockout/knock-in), CRISPRi/a (silencing/activation), Base Editor (base editing), Primer Editor (primer editing), and Epigenetic Regulation.

Various Cell Applications

With highly efficient and stable cell transfection and gene editing systems, Porton Advanced has successfully achieved gene editing in different cell lines, including but not limited to T cells, TIL cells, NK cells, iPSCs, and HSPCs.

Deliverables

Stable Cell Line •Various forms of delivery such as RNP, lentivirus, plasmids, and multiple detection methods
• Detailed delivery reports
Report •Includes relevant sequences, experimental records, and detection results.