Pre-made and Custom-Made mRNA and circRNA
Introduction
mRNA therapeutics hold the potential to transform modern medicine by providing a CGT platform with the capacity for rapid development and wide-scale deployment. Compared to recombinant proteins expressed in mammalian cell lines, manufacturing of mRNA is faster and more flexible as it can be easily produced by in vitro transcription.
Porton Advanced provides codon optimization, custom-made mRNA, custom-made circular RNA, and premade mRNA with options: capping, modified nucleotides, or lipid nanoparticle (LNP). Our codon optimization includes comprehensive consideration of RNA secondary structure, GC content and distribution, repeated sequences, and codon usage frequency. Our team of experts is dedicated to collaborating closely with you, ensuring that every RNA molecule we provide aligns seamlessly with your research objectives.
Our circRNA molecules are meticulously crafted to accelerate your research breakthroughs. Unlock the potential of circRNA and illuminate new pathways in your scientific inquiries. Our ready-to-use IVT mRNA can be used as a control for your mRNA experiment, or to assess the efficiency of mRNA delivery systems. For research-grade custom IVT mRNA preparation, please check our CRO services below. For scalable manufacturing of mRNA drugs, please check out our CDMO services.
Product
Product | Nucleotide | Scale |
EGFP IVT mRNA with cap1 | Unmodified | 100 ug,500 ug,1 mg |
m1Ψ substitution | 100 ug,500 ug,1 mg | |
mCherry IVT mRNA with cap1 | Unmodified | 100 ug,500 ug,1 mg |
m1Ψ substitution | 100 ug,500 ug,1 mg | |
Luciferase IVT mRNA with cap1 | Unmodified | 100 ug,500 ug,1 mg |
m1Ψ substitution | 100 ug,500 ug,1 mg | |
Cas9 IVT mRNA with cap1 | Unmodified | 100 ug,500 ug,1 mg |
m1Ψ substitution | 100 ug,500 ug,1 mg | |
EGFP circRNA | Unmodified | 100 ug,500 ug,1 mg |
mCherry circRNA | Unmodified | 100 ug,500 ug,1 mg |
Luciferase circRNA | Unmodified | 100 ug,500 ug,1 mg |
mRNA Case studies
- mRNA Integrity by CGE
- mRNA Capping Ratio by LC – MS
- mRNA-LNP Particle Size Distribution by SEC-MALS
- LNP-circRNA transfection efficiency and biocompatibility
Porton Advanced’s Analytical platform utilized the CGE method for assessing mRNA integrity. Results showed a high level of mRNA intactness, meeting the process development standard.
![](https://portonadvanced.com/wp-content/uploads/2023/11/d69ff310-55f1-42bd-afbf-39cbdde066cf.png)
Porton Advanced’s Analytical platform utilized the CGE method for assessing mRNA integrity. Results showed a high level of mRNA intactness, meeting the process development standard.
![](https://portonadvanced.com/wp-content/uploads/2023/11/cddf3720-3eb4-4ee2-ae10-f6403549e131.png)
Porton Advanced’s analytical platform showed the results on particle size distribution of LNP sample by SEC-MALS to be less than 0.1, proving the stable process delivered by the process development team.
![](https://portonadvanced.com/wp-content/uploads/2023/11/c4c3a50f-e460-47b0-b9f0-2069eeec801e.png)
![figure](https://portonadvanced.com/wp-content/uploads/2024/08/figure-1.jpg)
Compared to Lipo8000, LNP-circRNA showed higher transfection efficiency and biocompatibility. Through different routes of administration (intramuscular injection and intravenous injection), LNP-circRNA shows consistency in transfection efficiency in mice.
Workflow
![A diagram showing the process of making a vaccine.](https://portonadvanced.com/wp-content/uploads/2023/10/插画-3.png)
Quality control (QC)
Attribute | Method | Research Grade | |
General | Appearance | Visual inspection | √ |
pH | Potentiometric analysis | Optional | |
Identity | mRNA length | AGE | √ |
mRNA sequence | Sanger sequencing | √ | |
Content | mRNA concentration | UV spectrophotometry | √ |
Purity | A260/A280 | UV spectrophotometry | √ |
mRNA integrity | AGE | √ | |
CGE | √ | ||
Capping efficiency | LC-MS | Optional | |
PolyA tail length,distribution | LC-MS | Optional | |
Impurity | Residual DNA template | qPCR | Optional |
Residual protein | Micro BCA | Optional | |
dsRNA | ELISA | Optional | |
Residual cap analog | RP-HPLC | Optional | |
Residual NTPs | RP-HPLC | Optional | |
Potency | Gene expression | Western blot | Optional |
Cell transfection | Optional | ||
Safety | Endotoxin | Gel-clot / Photometric technique | Optional |
Sterility | Membrane filtration | Optional |
Deliverables
- IVT mRNA Product
- Certificate of Analysis (CoA)
Storage
Our mRNA products are stored in Tris buffer (pH 7.4) and can be stored at -80°C for up to 12 months. Both products are shipped on dry ice and should not be subjected to frequent freeze-thaw cycles.