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Vector Cloning Service
Plasmid Preparation Services
Vector Cloning Service
Introduction
Porton Advanced provides custom DNA vector cloning tailored to your specific research needs and offers a cost-effective and robust vector cloning platform with a fast turnaround time. We also offer plasmid DNA preparation, and virus packaging (lentivirus and AAV).
Services
Porton Advanced provides vector construction for shRNA vectors, gRNA vectors, Gene expression vectors, and more….
Workflow
- Day 1-2: Preparation and handling of original vectors and fragments (Note: Gene synthesis needs additional turnaround time))
- Day 3: Ligation and transformation
- Day 4: Cultivation of transformants
- Day 5-7: Positive clone screening and QC validation
Note: This demonstrates a regular turnaround time for vector construction if everything goes smoothly.
Deliverables
We offer complimentary small quantities of plasmid DNAs. Moreover, we provide a diverse range of high-quality plasmid DNA options for purchase, including Miniprep (>25 μg), Midiprep (>350 μg), Maxiprep (>1.2 mg), Megaprep (>5 mg), and Gigaprep (>15 mg).
For customers in need of viral vectors (lentivirus or AAV), we offer virus packaging for LV or AAV.
Quality Control (QC)
Our vectors undergo rigorous quality control procedures. These include thorough DNA quantification, A260/280 measurement, electrophoresis analysis of both undigested and restriction enzyme-digested plasmid DNAs, and comprehensive Sanger sequencing. These stringent QC measures ensure that our vectors maintain their integrity.
Plasmid Preparation Services
Introduction
Porton Advanced has developed plasmid DNA preparation services, designed to provide rapid and cost-efficient delivery of high-quality DNAs. Our plasmid preparation services cover a broad spectrum, accommodating both scientific researchers and industrial clients, with quantities ranging from micrograms to grams. Our offerings not only to research-oriented projects but also extend to manufacturing GMP-like and GMP-grade plasmid DNAs suitable for preclinical and clinical applications. With a commitment to excellence, we are proud to meet the diverse needs of our customers in the academic, biotech, and pharmaceutical communities.
Services
| Scale | Application | Deliverable（Plasmid DNA） | Time |
| Miniprep | Molecular biology | 25 µg, >100 ng/µL | 1 – 2 days |
| Midiprep |
Molecular biology and cell culture (endotoxin-free, sterile) |
350 µg, >500 ng/µL | 3 – 4 days |
| Maxiprep | 1.2 mg, >500 ng/µL | ||
| Megaprep | 5 mg, >500 ng/µL | ||
| Gigaprep | 15 mg, >500 ng/µL | 5 – 8 days | |
| Industrial Grade | Research and preclinical applications | 20 mg to 1 g | 1 – 2 weeks |
Workflow
Example: Maxiprep Plasmid Scale
- Day 1-2: Transform plasmid into E. coli and prepare bacterial fluids for plasmid extraction
- Day 3: Prepare extraction and purification of plasmid DNAs
- Day 4-5: Perform quality control of plasmid DNAs
Quality Control (QC)
Our comprehensive quality control (QC) for plasmid DNAs includes DNA quantification, A260/280 and A260/230 measurements, restriction enzyme digestion, Sanger sequencing. For Maxiprep and above, our plasmid preparation services can achieve higher supercoiled content (90% ± 10%) with lower endotoxin levels (<0.01 EU/μg). Additionally, we offer animal-free plasmid preparation upon request.
Deliverables
- Plasmid DNAs
- Certificate of Analysis (CoA)
Storage condition
Our plasmid DNAs is stored in 1 × TE buffer by default (However, Ultra-purified H2O upon request). The plasmid DNA can be stored at – 80 °C for up to 12-24 months.
Note: Please avoid freeze-thaw cycles.