Engineering
Stable Cell Line Engineering
We offer customized editing solutions suitable for various cell scenarios, while also supporting diverse delivery methods such as RNP, lentivirus, plasmids, and multiple detection methods. This ensures efficient and stable cell lines in addition to comprehensive testing services.
Services
Gene knock-out stable cell line construction, gene knock-in stable cell line construction, and point mutation stable cell line construction.
Gene Editing Design
- Customized editing schemes provided through multi-dimensional evaluation
- Design of sgRNA, donor DNA, and relevant vectors
Cell Culture and Transfection
- Applicable to various cell scenarios
- Diverse delivery methods
Identification and Detection of Transfected Cells
- Multi-dimensional evaluation of editing efficiency
- Multi-angle detection of cell function
Cell Line Delivery
- Various forms of delivery such as RNP, lentivirus, plasmids, and multiple detection methods
- Detailed delivery reports
Workflow
CRISPR/Cas nucleases Gene Editing System
Cas9/cas12 (knockout/knock-in), CRISPRi/a (silencing/activation), Base Editor (base editing), Primer Editor (primer editing), and Epigenetic Regulation.
Custom Services for Varying Types of Cell Line Generation
Gene knock-out (KO) cell line generation by CRISPR
| Type | Turnaround time | Price |
| Single KO pool | 6-9 weeks | $2,999 and up |
| Single KO clone (frameshift) | 10-14 weeks | $3,899 and up |
| Single KO clone (deletion) | 10-14 weeks | $6,799 and up |
| Double KO pool（frameshift) | Please inquire | |
| Double KO clone (frameshift) | Please inquire | |
| Double KO pool（deletion) | Please inquire | |
| Double KO clone (deletion) | Please inquire | |
Note: For cell pool, we deliver 2 vials with >10E6 cells/vial. For single clone, we also deliver 2 vials with >10E6 cells/vial.
Gene knock-in (KI) cell line generation by CRISPR
| Type | Turnaround time | Price |
| Cell pool (fragment < 3kb) | 8-12 weeks | $5,599 |
| Single clone (fragment < 3kb) | 14-18 weeks | $8,599 |
| Cell pool (fragment > 3kb) | 10-16 weeks | $7,999 |
| Single clone (fragment > 3kb) | 16-22 weeks | $11,599 |
| Double KI pool | Please inquire | |
| Double KI clone | Please inquire | |
| Triple KI pool | Please inquire | |
| Triple KI clone | Please inquire | |
Note: Typically, we provide one clone cell lines. If your project requires two clones, please contact our sales team.
Cell line generation by Base Editor, Primer Editor, CRISPR i/a, or Point Mutation
| Type | Turnaround time | Price |
| Cell pool | 8-12 weeks | $5,999 and up |
| Single clone | 14-18 weeks | $8,599 and up |
Quality Control (QC) Testing and Analysis Includes:
- Verification using sanger sequencing, mycoplasma testing, and sterility testing
- Analysis of off-target effects (Sanger Sequencing, NGS)
- Phenotypic analysis and functional validation tests (Cell Proliferation, Apoptosis, Migration, Cell Viability, Cytotoxicity, etc.)
- The following tests can be completed at additional cost as needed:
- Expression tests such as RT-qPCR, WB, and FACS
Gene overexpression stable cell line (using lentivirus)
| Type | Turnaround time | Price |
| Cell pool | 6-10 weeks | $2,899 and up |
| Single clone | 12-18 weeks | $3,899 and up |
Note: For cell pool, we deliver 2 vials with >10E6 cells/vial.
Quality Control (QC) Testing and Analysis Includes:
- Expression tests (RT-qPCR, mycoplasma testing, and sterility testing)
- The following tests can be completed at additional cost as needed:
- Expression tests (WB, FACS)
- Analysis of off-target effects (Sanger Sequencing, NGS)
- Phenotypic analysis and functional validation tests (Cell Proliferation, Apoptosis, Migration, Cell Viability, Cytotoxicity, etc.)
With highly efficient and stable cell transfection and gene editing systems, Porton Advanced has successfully achieved gene editing in different cell lines, including but not limited to T cells, TIL cells, NK cells, iPSCs, and HSPCs.
Deliverables
| Stable Cell Line | •Various forms of delivery such as RNP, lentivirus, plasmids, and multiple detection methods |
| • Detailed delivery reports | |
| Report | •Includes relevant sequences, experimental records, and detection results. |