mRNA and circRNA

Pre-made and Custom-Made mRNA and circRNA

Introduction

mRNA therapeutics hold the potential to transform modern medicine by providing a CGT platform with the capacity for rapid development and wide-scale deployment. Compared to recombinant proteins expressed in mammalian cell lines, manufacturing of mRNA is faster and more flexible as it can be easily produced by in vitro transcription.

Porton Advanced provides codon optimization, custom-made mRNA, custom-made circular RNA, and premade mRNA with options: capping, modified nucleotides, or lipid nanoparticle (LNP). Our codon optimization includes comprehensive consideration of RNA secondary structure, GC content and distribution, repeated sequences, and codon usage frequency. Our team of experts is dedicated to collaborating closely with you, ensuring that every RNA molecule we provide aligns seamlessly with your research objectives.

Our circRNA molecules are meticulously crafted to accelerate your research breakthroughs. Unlock the potential of circRNA and illuminate new pathways in your scientific inquiries. Our ready-to-use IVT mRNA can be used as a control for your mRNA experiment, or to assess the efficiency of mRNA delivery systems. For research-grade custom IVT mRNA preparation, please check our CRO services below. For scalable manufacturing of mRNA drugs, please check out our CDMO services.

Product

Custom-made mRNA with the option of Capping, Modified Nucleotides, or Lipid Nanoparticles (LNPs)
Experience the epitome of precision with our custom-made mRNA solutions. Tailor your experiments with advanced capping (Cap1) and modified nucleotide options (N1-Methylpseudouridine-m1Ψ, 5-methylcytosine-m5C, etc.) or Lipid Nanoparticle (LNP), enhancing mRNA stability and translational efficiency.

Custom-Made circRNA
Delve into the world of circular RNA with our custom-made circRNA products. Circular RNAs have emerged as pivotal players in gene regulation, and our circRNA solutions are engineered to empower your investigations.

Pre-made mRNA with the option of capping, Modified Nucleotides
Our premade mRNA (EGFP/ mCherry/ Luciferase/ Cas9) was generated with cap1 and with or without modified nucleotide, N1-Methylpseudouridine (m1Ψ). Both the protein expression (EGFP/ mCherry/ Luciferase) and the editing function (Cas9) of our pre-made mRNA have been fully validated for high levels in the cell culture.

Product	Nucleotide	Scale
EGFP IVT mRNA with cap1	Unmodified	100 ug，500 ug，1 mg
EGFP IVT mRNA with cap1	m1Ψ substitution	100 ug，500 ug，1 mg
mCherry IVT mRNA with cap1	Unmodified	100 ug，500 ug，1 mg
mCherry IVT mRNA with cap1	m1Ψ substitution	100 ug，500 ug，1 mg
Luciferase IVT mRNA with cap1	Unmodified	100 ug，500 ug，1 mg
Luciferase IVT mRNA with cap1	m1Ψ substitution	100 ug，500 ug，1 mg
Cas9 IVT mRNA with cap1	Unmodified	100 ug，500 ug，1 mg
Cas9 IVT mRNA with cap1	m1Ψ substitution	100 ug，500 ug，1 mg
EGFP circRNA	Unmodified	100 ug，500 ug，1 mg
mCherry circRNA	Unmodified	100 ug，500 ug，1 mg
Luciferase circRNA	Unmodified	100 ug，500 ug，1 mg

mRNA Case studies

Porton Advanced’s Analytical platform utilized the CGE method for assessing mRNA integrity. Results showed a high level of mRNA intactness, meeting the process development standard.

*（source: Porton Advanced’s Analytical Development Platform）*

Porton Advanced’s Analytical platform utilized the CGE method for assessing mRNA integrity. Results showed a high level of mRNA intactness, meeting the process development standard.

Porton Advanced’s analytical platform showed the results on particle size distribution of LNP sample by SEC-MALS to be less than 0.1, proving the stable process delivered by the process development team.

Compared to Lipo8000, LNP-circRNA showed higher transfection efficiency and biocompatibility. Through different routes of administration (intramuscular injection and intravenous injection), LNP-circRNA shows consistency in transfection efficiency in mice.

Workflow

A diagram showing the process of making a vaccine.

Quality control (QC)

Attribute		Method	Research Grade
General	Appearance	Visual inspection	√
General	pH	Potentiometric analysis	Optional
Identity	mRNA length	AGE	√
Identity	mRNA sequence	Sanger sequencing	√
Content	mRNA concentration	UV spectrophotometry	√
Purity	A260/A280	UV spectrophotometry	√
	mRNA integrity	AGE	√
	mRNA integrity	CGE	√
	Capping efficiency	LC-MS	Optional
	PolyA tail length,distribution	LC-MS	Optional
Impurity	Residual DNA template	qPCR	Optional
	Residual protein	Micro BCA	Optional
	dsRNA	ELISA	Optional
	Residual cap analog	RP-HPLC	Optional
	Residual NTPs	RP-HPLC	Optional
Potency	Gene expression	Western blot	Optional
Potency	Gene expression	Cell transfection	Optional
Safety	Endotoxin	Gel-clot / Photometric technique	Optional
Safety	Sterility	Membrane filtration	Optional

Deliverables

IVT mRNA Product
Certificate of Analysis (CoA)

Storage

Our mRNA products are stored in Tris buffer (pH 7.4) and can be stored at -80°C for up to 12 months. Both products are shipped on dry ice and should not be subjected to frequent freeze-thaw cycles.